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'Marvan R, Mazura I, De Ruiter JR, 2006: A Report on Paternity Testing in Bonobos (Pan Paniscus): Difficulty of Obtaining Reliable Genotypes by Analysis of Ca/Gt Repetitive Sequences. Anthropologie (Brno) 44, 2: 201-207'. |
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| Abstract | | The paper shows that nuclear DNA extracted from plucked hairs can be used for the amplification of dinucleotide short tandem repeats (microsatellites). Five polymorphic microsatellite loci with CA/GT repeat units for paternity testing in a captive group of bonobos (Pan paniscus) were investigated. All PCR reactions performed yielded positive products at the first attempt. DNA extracted from hair is present in a small amount and is usually degraded. Therefore PCR products can often be easily obtained but the genotypes can be unreliable. Also in case of the present analysis, stutter bands (false alleles) were generated, making the assignment of true alleles difficult. It is concluded that these false alleles were results of reaction conditions during the amplification process. Methods used to determine the genetic relationships as well as possible consequences of the project for breeding management of the bonobo group are presented and discussed. | | | | Keywords | | Pan paniscus - Hair samples - PCR - Microsatellite genotyping - DNA slippage | | |
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