ANTHROPOLOGIE
International Journal of Human Diversity and Evolution
 
Coverage: 1923-1941 (Vols. I-XIX) & 1962-2019 (Vols. 1-57)
ISSN 0323-1119 (Print)
ISSN 2570-9127 (Online)
News:
It is with deep regret and profound sadness that we inform all colleagues: Doc. MUDr. Vladimír Novotný, CSc, a long-time member of the editorial board of the Anthropologie, has died on 30th November 2019 at the age of 80 years.
World Archaeological Congres 9
 

 
 
 
 
 
 
 
SCImago Journal & Country Rank
 
 
Full text of article
'Marvan R, Mazura I, De Ruiter JR, 2006: A Report on Paternity Testing in Bonobos (Pan Paniscus): Difficulty of Obtaining Reliable Genotypes by Analysis of Ca/Gt Repetitive Sequences. Anthropologie (Brno) 44, 2: 201-207'.
 
Abstract
The paper shows that nuclear DNA extracted from plucked hairs can be used for the amplification of dinucleotide short tandem repeats (microsatellites). Five polymorphic microsatellite loci with CA/GT repeat units for paternity testing in a captive group of bonobos (Pan paniscus) were investigated. All PCR reactions performed yielded positive products at the first attempt. DNA extracted from hair is present in a small amount and is usually degraded. Therefore PCR products can often be easily obtained but the genotypes can be unreliable. Also in case of the present analysis, stutter bands (false alleles) were generated, making the assignment of true alleles difficult. It is concluded that these false alleles were results of reaction conditions during the amplification process. Methods used to determine the genetic relationships as well as possible consequences of the project for breeding management of the bonobo group are presented and discussed.
 
Keywords
Pan paniscus - Hair samples - PCR - Microsatellite genotyping - DNA slippage
 
 
 
 

 Full text (PDF)

 Export citation

 Related articles